2F3
| Antibody ID | AN0006 |
|---|
| Epitope ID | EP0012 |
|---|---|
| Epitope name | Sialyl Lewis x |
| Recognition region | Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)[HSO3(-6)]Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)][HSO3(-6)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)[HSO3(-6)]Gal(b1-4)[Fuc(a1-3)][HSO3(-6)]GlcNAc(b1-3)Gal(b1-)-R |
| Immunoprecipitation | |
| Immunoblot | [3] |
| ELISA/RIA | |
| Flow cytometry | |
| Histochemistry | [1][2] |
| Comment |
| Epitope ID | EP0014 |
|---|---|
| Epitope name | 6'-Sulfo Sialyl Lewis x |
| Recognition region | Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)[HSO3(-6)]Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)][HSO3(-6)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)[HSO3(-6)]Gal(b1-4)[Fuc(a1-3)][HSO3(-6)]GlcNAc(b1-3)Gal(b1-)-R |
| Immunoprecipitation | |
| Immunoblot | |
| ELISA/RIA | |
| Flow cytometry | |
| Histochemistry | [1][2] |
| Comment |
| Epitope ID | EP0015 |
|---|---|
| Epitope name | Sialyl 6-Sulfo Lewis x |
| Recognition region | Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)[HSO3(-6)]Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)][HSO3(-6)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)[HSO3(-6)]Gal(b1-4)[Fuc(a1-3)][HSO3(-6)]GlcNAc(b1-3)Gal(b1-)-R |
| Immunoprecipitation | |
| Immunoblot | |
| ELISA/RIA | |
| Flow cytometry | |
| Histochemistry | [1][2] |
| Comment |
| Epitope ID | EP0016 |
|---|---|
| Epitope name | 6,6'- Disulfo Sialyl Lewis x |
| Recognition region | Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)[HSO3(-6)]Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)][HSO3(-6)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)[HSO3(-6)]Gal(b1-4)[Fuc(a1-3)][HSO3(-6)]GlcNAc(b1-3)Gal(b1-)-R |
| Immunoprecipitation | |
| Immunoblot | |
| ELISA/RIA | |
| Flow cytometry | |
| Histochemistry | [1] |
| Comment |
| Species | Mouse |
|---|---|
| Category | Monoclonal |
| Isotype | IgM |
| Immunogen | sialyl Lewis x glycolipid（IV3Neu5AcIII3FucnLc4Cer)[3] |
| Availability | |
| Applications |
| 1 | Renkonen J, Tynninen O, Hayry P, Paavonen T, Renkonen R. Glycosylation might provide endothelial zip codes for organ-specific leukocyte traffic into inflammatory sites. Am J Pathol. 2002 Aug ;161(2):543-50 PMID: 12163379 |
|---|---|
| 2 | Wagers AJ, Stoolman LM, Kannagi R, Craig R, Kansas GS. Expression of leukocyte fucosyltransferases regulates binding to E-selectin: relationship to previously implicated carbohydrate epitopes. J Immunol. 1997 Aug 15;159(4):1917-29 PMID: 9257857 |
| 3 | Ohmori K, Takada A, Ohwaki I, Takahashi N, Furukawa Y, Maeda M, Kiso M, Hasegawa A, Kannagi M, Kannagi R. A distinct type of sialyl Lewis X antigen defined by a novel monoclonal antibody is selectively expressed on helper memory T cells. Blood. 1993 Nov 1;82(9):2797-805 PMID: 7693048 |