2H5
| Antibody ID | AN0007 |
|---|
| Epitope ID | EP0012 |
|---|---|
| Epitope name | Sialyl Lewis x |
| Recognition region | Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)[HSO3(-6)]Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)][HSO3(-6)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)[HSO3(-6)]Gal(b1-4)[Fuc(a1-3)][HSO3(-6)]GlcNAc(b1-3)Gal(b1-)-R |
| Immunoprecipitation | [1] |
| Immunoblot | [8][2][6][9][7] |
| ELISA/RIA | |
| Flow cytometry | |
| Histochemistry | [3][5][10][2][11][4*] |
| Comment |
| Epitope ID | EP0014 |
|---|---|
| Epitope name | 6'-Sulfo Sialyl Lewis x |
| Recognition region | Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)[HSO3(-6)]Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)][HSO3(-6)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)[HSO3(-6)]Gal(b1-4)[Fuc(a1-3)][HSO3(-6)]GlcNAc(b1-3)Gal(b1-)-R |
| Immunoprecipitation | [1] |
| Immunoblot | [8][2][6][9][7] |
| ELISA/RIA | |
| Flow cytometry | |
| Histochemistry | [3][5][10][2][11][4*] |
| Comment |
| Epitope ID | EP0015 |
|---|---|
| Epitope name | Sialyl 6-Sulfo Lewis x |
| Recognition region | Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)[HSO3(-6)]Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)][HSO3(-6)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)[HSO3(-6)]Gal(b1-4)[Fuc(a1-3)][HSO3(-6)]GlcNAc(b1-3)Gal(b1-)-R |
| Immunoprecipitation | [1] |
| Immunoblot | [8][2][6][9][7] |
| ELISA/RIA | |
| Flow cytometry | |
| Histochemistry | [3][5][10][2][11][4*] |
| Comment |
| Epitope ID | EP0016 |
|---|---|
| Epitope name | 6,6'- Disulfo Sialyl Lewis x |
| Recognition region | Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)[HSO3(-6)]Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)][HSO3(-6)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)[HSO3(-6)]Gal(b1-4)[Fuc(a1-3)][HSO3(-6)]GlcNAc(b1-3)Gal(b1-)-R |
| Immunoprecipitation | [1] |
| Immunoblot | [8][2][6][9][7] |
| ELISA/RIA | |
| Flow cytometry | |
| Histochemistry | [3][5][10][2][11][4*] |
| Comment |
| Species | Mouse |
|---|---|
| Category | Monoclonal |
| Isotype | IgM |
| Immunogen | sialyl Lewis x-active glycolipids[8] |
| Availability | SEIKAGAKU BIOBUSINESS CORPORATION -English SEIKAGAKU BIOBUSINESS CORPORATION -Japanese |
| Applications |
| 1 | Tamatani T, Suematsu M, Tezuka K, Hanzawa N, Tsuji T, Ishimura Y, Kannagi R, Toyoshima S, Homma M. Recognition of consensus CHO structure in ligands for selectins by novel antibody against sialyl Lewis X. Am J Physiol. 1995 Oct ;269(4 Pt 2):H1282-7 PMID: 7485559 |
|---|---|
| 2 | Akahori T, Yuzawa Y, Nishikawa K, Tamatani T, Kannagi R, Miyasaka M, Okada H, Hotta N, Matsuo S. Role of a sialyl Lewis(x)-like epitope selectively expressed on vascular endothelial cells in local skin inflammation of the rat. J Immunol. 1997 Jun 1;158(11):5384-92 PMID: 9164959 |
| 3 | Takaeda M, Yokoyama H, Segawa-Takaeda C, Wada T, Kobayashi K. High endothelial venule-like vessels in the interstitial lesions of human glomerulonephritis. Am J Nephrol. 2002 Jan ;22(1):48-57 PMID: 11919403 |
| 4 | Mitsuoka C, Sawada-Kasugai M, Ando-Furui K, Izawa M, Nakanishi H, Nakamura S, Ishida H, Kiso M, Kannagi R. Identification of a major carbohydrate capping group of the L-selectin ligand on high endothelial venules in human lymph nodes as 6-sulfo sialyl Lewis X. J Biol Chem. 1998 May 1;273(18):11225-33 PMID: 9556613 |
| 5 | Wagers AJ, Stoolman LM, Kannagi R, Craig R, Kansas GS. Expression of leukocyte fucosyltransferases regulates binding to E-selectin: relationship to previously implicated carbohydrate epitopes. J Immunol. 1997 Aug 15;159(4):1917-29 PMID: 9257857 |
| 6 | Seko Y, Enokawa Y, Tamatani T, Kannagi R, Yagita H, Okumura K, Yazaki Y. Expression of sialyl Lewis(X) in rat heart with ischaemia/reperfusion and reduction of myocardial reperfusion injury by a monoclonal antibody against sialyl Lewis(X) J Pathol. 1996 Nov ;180(3):305-10 PMID: 8958810 |
| 7 | Kimura N, Mitsuoka C, Kanamori A, Hiraiwa N, Uchimura K, Muramatsu T, Tamatani T, Kansas GS, Kannagi R. Reconstitution of functional L-selectin ligands on a cultured human endothelial cell line by cotransfection of alpha1-->3 fucosyltransferase VII and newly cloned GlcNAcbeta:6-sulfotransferase cDNA. Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4530-5 PMID: 10200296 |
| 8 | Sawada M, Takada A, Ohwaki I, Takahashi N, Tateno H, Sakamoto J, Kannagi R. Specific expression of a complex sialyl Lewis X antigen on high endothelial venules of human lymph nodes: possible candidate for L-selectin ligand. Biochem Biophys Res Commun. 1993 May 28;193(1):337-47 PMID: 7684905 |
| 9 | Clark RA, Fuhlbrigge RC, Springer TA. L-Selectin ligands that are O-glycoprotease resistant and distinct from MECA-79 antigen are sufficient for tethering and rolling of lymphocytes on human high endothelial venules. J Cell Biol. 1998 Feb 9;140(3):721-31 PMID: 9456330 |
| 10 | Suematsu M, Suzuki H, Tamatani T, Iigou Y, DeLano FA, Miyasaka M, Forrest MJ, Kannagi R, Zweifach BW, Ishimura Y, et al. Impairment of selectin-mediated leukocyte adhesion to venular endothelium in spontaneously hypertensive rats. J Clin Invest. 1995 Oct ;96(4):2009-16 PMID: 7560094 |
| 11 | Mitsuoka C, Kawakami-Kimura N, Kasugai-Sawada M, Hiraiwa N, Toda K, Ishida H, Kiso M, Hasegawa A, Kannagi R. Sulfated sialyl Lewis X, the putative L-selectin ligand, detected on endothelial cells of high endothelial venules by a distinct set of anti-sialyl Lewis X antibodies. Biochem Biophys Res Commun. 1997 Jan 23;230(3):546-51 PMID: 9015359 |