RL2 / RL-2
| Antibody ID | AN0385 |
|---|
| Epitope ID | EP0004 |
|---|---|
| Epitope name | O-GlcNAc |
| Recognition region | GlcNAc(b1-)Ser/Thr |
| Immunoprecipitation | [1][2][4][5] |
| Immunoblot | [3][6][7][8][9][4][10][11][12][13*][14][15][5][17] |
| ELISA/RIA | |
| Flow cytometry | |
| Histochemistry | [16] |
| Comment |
| Species | Mouse |
|---|---|
| Category | Monoclonal |
| Isotype | IgG1 |
| Immunogen | |
| Availability | abcam Affinity BioReagents Santa Cruz Biotechnology, Inc. |
| Applications |
| 1 | Holt GD, Snow CM, Senior A, Haltiwanger RS, Gerace L, Hart GW. Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine. J Cell Biol. 1987 May ;104(5):1157-64 PMID: 3571327 |
|---|---|
| 2 | Snow CM, Senior A, Gerace L. Monoclonal antibodies identify a group of nuclear pore complex glycoproteins. J Cell Biol. 1987 May ;104(5):1143-56 PMID: 2437126 |
| 3 | Walgren JL, Vincent TS, Schey KL, Buse MG. High glucose and insulin promote O-GlcNAc modification of proteins, including alpha-tubulin. Am J Physiol Endocrinol Metab. 2003 Feb ;284(2):E424-34 PMID: 12397027 |
| 4 | Guinez C, Lemoine J, Michalski JC, Lefebvre T. 70-kDa-heat shock protein presents an adjustable lectinic activity towards O-linked N-acetylglucosamine. Biochem Biophys Res Commun. 2004 Jun 18;319(1):21-6 PMID: 15158436 |
| 5 | Lefebvre T, Pinte S, Guerardel C, Deltour S, Martin-Soudant N, Slomianny MC, Michalski JC, Leprince D. The tumor suppressor HIC1 (hypermethylated in cancer 1) is O-GlcNAc glycosylated. Eur J Biochem. 2004 Oct ;271(19):3843-54 PMID: 15373830 |
| 6 | Buse MG, Robinson KA, Marshall BA, Hresko RC, Mueckler MM. Enhanced O-GlcNAc protein modification is associated with insulin resistance in GLUT1-overexpressing muscles. Am J Physiol Endocrinol Metab. 2002 Aug ;283(2):E241-50 PMID: 12110528 |
| 7 | Gao Y, Miyazaki J, Hart GW. The transcription factor PDX-1 is post-translationally modified by O-linked N-acetylglucosamine and this modification is correlated with its DNA binding activity and insulin secretion in min6 beta-cells. Arch Biochem Biophys. 2003 Jul 15;415(2):155-63 PMID: 12831837 |
| 8 | Zraika S, Dunlop M, Proietto J, Andrikopoulos S. The hexosamine biosynthesis pathway regulates insulin secretion via protein glycosylation in mouse islets. Arch Biochem Biophys. 2002 Sep 15;405(2):275-9 PMID: 12220542 |
| 9 | Andrali SS, Marz P, Ozcan S. Ataxin-10 interacts with O-GlcNAc transferase OGT in pancreatic beta cells. Biochem Biophys Res Commun. 2005 Nov 11;337(1):149-53 PMID: 16182253 |
| 10 | Sohn KC, Lee KY, Park JE, Do SI. OGT functions as a catalytic chaperone under heat stress response: a unique defense role of OGT in hyperthermia. Biochem Biophys Res Commun. 2004 Sep 24;322(3):1045-51 PMID: 15336570 |
| 11 | Cauet G, Strub JM, Leize E, Wagner E, Van Dorsselaer A, Lusky M. Identification of the glycosylation site of the adenovirus type 5 fiber protein. Biochemistry. 2005 Apr 12;44(14):5453-60 PMID: 15807538 |
| 12 | Cheng X, Cole RN, Zaia J, Hart GW. Alternative O-glycosylation/O-phosphorylation of the murine estrogen receptor beta. Biochemistry. 2000 Sep 26;39(38):11609-20 PMID: 10995228 |
| 13 | Gewinner C, Hart G, Zachara N, Cole R, Beisenherz-Huss C, Groner B. The coactivator of transcription CREB-binding protein interacts preferentially with the glycosylated form of Stat5. J Biol Chem. 2004 Jan 30;279(5):3563-72 PMID: 14597631 |
| 14 | Meyer BH, Luus HG, Muller FO, Badenhorst PN, Lotter MG, Rothig HJ, Grotsch H, Rosenkranz B. Recombinant hirudin, a new anticoagulant, has no effect on faecal blood loss. Br J Clin Pharmacol. 1992 May ;33(5):524-6 PMID: 1524967 |
| 15 | Cieniewski-Bernard C, Bastide B, Lefebvre T, Lemoine J, Mounier Y, Michalski JC. Identification of O-linked N-acetylglucosamine proteins in rat skeletal muscle using two-dimensional gel electrophoresis and mass spectrometry. Mol Cell Proteomics. 2004 Jun ;3(6):577-85 PMID: 14985449 |
| 16 | Hanover JA, Lai Z, Lee G, Lubas WA, Sato SM. Elevated O-linked N-acetylglucosamine metabolism in pancreatic beta-cells. Arch Biochem Biophys. 1999 Feb 1;362(1):38-45 PMID: 9917327 |
| 17 | Du XL, Edelstein D, Rossetti L, Fantus IG, Goldberg H, Ziyadeh F, Wu J, Brownlee M. Hyperglycemia-induced mitochondrial superoxide overproduction activates the hexosamine pathway and induces plasminogen activator inhibitor-1 expression by increasing Sp1 glycosylation. Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12222-6 PMID: 11050244 |