5F4
| Antibody ID | AN0069 |
|---|
| Epitope ID | EP0021 |
|---|---|
| Epitope name | Tn Antigen |
| Recognition region | GalNAc(a1-)Ser/Thr |
| Immunoprecipitation | |
| Immunoblot | |
| ELISA/RIA | |
| Flow cytometry | [2] |
| Histochemistry | [1][3] |
| Comment |
| Species | Mouse |
|---|---|
| Category | Monoclonal |
| Isotype | IgM |
| Immunogen | |
| Availability | |
| Applications |
| 1 | Thurnher M, Clausen H, Sharon N, Berger EG. Use of O-glycosylation-defective human lymphoid cell lines and flow cytometry to delineate the specificity of Moluccella laevis lectin and monoclonal antibody 5F4 for the Tn antigen (GalNAc alpha 1-O-Ser/Thr). Immunol Lett. 1993 Jun ;36(3):239-43 PMID: 8370596 |
|---|---|
| 2 | Ragupathi G, Liu NX, Musselli C, Powell S, Lloyd K, Livingston PO. Antibodies against tumor cell glycolipids and proteins, but not mucins, mediate complement-dependent cytotoxicity. J Immunol. 2005 May 1;174(9):5706-12 PMID: 15843572 |
| 3 | Rajpert-De Meyts E, Poll SN, Goukasian I, Jeanneau C, Herlihy AS, Bennett EP, Skakkebaek NE, Clausen H, Giwercman A, Mandel U. Changes in the profile of simple mucin-type O-glycans and polypeptide GalNAc-transferases in human testis and testicular neoplasms are associated with germ cell maturation and tumour differentiation. Virchows Arch. 2007 Oct ;451(4):805-14 PMID: 17694322 |