A2B5
| Antibody ID | AN0214 |
|---|
| Epitope ID | EP0032 |
|---|---|
| Epitope name | C-Series Gangliosides Oligosaccharide / Hemato- or Ganglio-Type |
| Recognition region | Neu5Ac(a2-8)Neu5Ac(a2-8)Neu5Ac(a2-3)Gal-R Neu5Ac9Ac(a2-8)Neu5Ac(a2-8)Neu5Ac(a2-3)Gal-R |
| Immunoprecipitation | |
| Immunoblot | [1*][2][3][4][5] |
| ELISA/RIA | |
| Flow cytometry | |
| Histochemistry | |
| Comment |
| Epitope ID | EP0033 |
|---|---|
| Epitope name | C-Series Gangliosides Oligosaccharide / Gangliotetraosyl Core Structure |
| Recognition region | Neu5Ac(a2-8)Neu5Ac(a2-8)Neu5Ac(a2-3)Gal-R Neu5Ac9Ac(a2-8)Neu5Ac(a2-8)Neu5Ac(a2-3)Gal-R |
| Immunoprecipitation | |
| Immunoblot | [1*][2][3][4][5] |
| ELISA/RIA | |
| Flow cytometry | |
| Histochemistry | |
| Comment |
| Epitope ID | EP0070 |
|---|---|
| Epitope name | GT3 |
| Recognition region | Neu5Ac(a2-8)Neu5Ac(a2-8)Neu5Ac(a2-3)Gal-R Neu5Ac9Ac(a2-8)Neu5Ac(a2-8)Neu5Ac(a2-3)Gal-R |
| Immunoprecipitation | |
| Immunoblot | [1*][2][3] |
| ELISA/RIA | |
| Flow cytometry | |
| Histochemistry | |
| Comment |
| Species | Mouse |
|---|---|
| Category | Monoclonal |
| Isotype | IgM |
| Immunogen | |
| Availability | ATCC |
| Applications |
| 1 | Dubois C, Manuguerra JC, Hauttecoeur B, Maze J. Monoclonal antibody A2B5, which detects cell surface antigens, binds to ganglioside GT3 (II3 (NeuAc)3LacCer) and to its 9-O-acetylated derivative. J Biol Chem. 1990 Feb 15;265(5):2797-803 PMID: 2303428 |
|---|---|
| 2 | Saito M, Ito M, Sugiyama K. A specific loss of C-series gangliosides in pancreas of streptozotocin-induced diabetic rats. Life Sci. 1999 ;64(20):1803-10 PMID: 10350354 |
| 3 | Saito M, Sugiyama K. A distinct ganglioside composition of rat pancreatic islets. Arch Biochem Biophys. 2000 Apr 15;376(2):371-6 PMID: 10775425 |
| 4 | Saito M, Kitamura H, Sugiyama K. The specificity of monoclonal antibody A2B5 to c-series gangliosides. J Neurochem. 2001 Jul ;78(1):64-74 PMID: 11432974 |
| 5 | Nakamura K, Kojima H, Suzuki M, Suzuki A, Tamai Y. Novel polysialogangliosides of skate brain structural determination of tetra, penta and hexasialogangliosides with a NeuAc-GalNAc linkage. Eur J Biochem. 2000 Aug ;267(16):5198-208 PMID: 10931205 |