FH7 / FH-7
| Antibody ID | AN0259 |
|---|
| Epitope ID | EP0040 |
|---|---|
| Epitope name | Disialyl Lewis a |
| Recognition region | Neu5Ac(a2-3)Gal(b1-3)[Neu5Ac(a2-6)][Fuc(a1-4)]GlcNAc(b1-3)Gal(b1-)-R |
| Immunoprecipitation | |
| Immunoblot | [1*][2*] |
| ELISA/RIA | [3] |
| Flow cytometry | |
| Histochemistry | |
| Comment |
| Species | Mouse |
|---|---|
| Category | Monoclonal |
| Isotype | IgG3 |
| Immunogen | |
| Availability | Millipore |
| Applications |
| 1 | Nudelman E, Fukushi Y, Levery SB, Higuchi T, Hakomori S. Novel fucolipids of human adenocarcinoma: disialosyl Lea antigen (III4FucIII6NeuAcIV3NeuAcLc4) of human colonic adenocarcinoma and the monoclonal antibody (FH7) defining this structure. J Biol Chem. 1986 Apr 25;261(12):5487-95 PMID: 3957932 |
|---|---|
| 2 | Tsuchida A, Okajima T, Furukawa K, Ando T, Ishida H, Yoshida A, Nakamura Y, Kannagi R, Kiso M, Furukawa K. Synthesis of disialyl Lewis a (Le(a)) structure in colon cancer cell lines by a sialyltransferase, ST6GalNAc VI, responsible for the synthesis of alpha-series gangliosides. J Biol Chem. 2003 Jun 20;278(25):22787-94 PMID: 12668675 |
| 3 | Zenita K, Hirashima K, Shigeta K, Hiraiwa N, Takada A, Hashimoto K, Fujimoto E, Yago K, Kannagi R. Northern hybridization analysis of VH gene expression in murine monoclonal antibodies directed to cancer-associated ganglioside antigens having various sialic acid linkages. J Immunol. 1990 Jun 1;144(11):4442-51 PMID: 2111348 |