FH6 / FH-6
| Antibody ID | AN0263 |
|---|
| Epitope ID | EP0012 |
|---|---|
| Epitope name | Sialyl Lewis x |
| Recognition region | Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-4)GlcNAc(b1-3)Gal(b1-)-R |
| Immunoprecipitation | [1] |
| Immunoblot | [4*][6] |
| ELISA/RIA | [7][3] |
| Flow cytometry | [5] |
| Histochemistry | [2][7] |
| Comment |
| Epitope ID | EP0043 |
|---|---|
| Epitope name | Sialyl Lewis x-i |
| Recognition region | Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-)-R Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-3)Gal(b1-4)GlcNAc(b1-3)Gal(b1-)-R |
| Immunoprecipitation | [1] |
| Immunoblot | [6] |
| ELISA/RIA | [7][3] |
| Flow cytometry | |
| Histochemistry | [2][7] |
| Comment |
| Species | Mouse |
|---|---|
| Category | Monoclonal |
| Isotype | IgM |
| Immunogen | |
| Availability | ATCC |
| Applications | The monoclonal antibody,FH-6 is representative diagnostic agent for adenocarcinoma. |
| 1 | Symington FW, Holmes EH, Symington BE. Human epidermal keratinocyte expression of sialyl-Lewis X. J Invest Dermatol. 1992 Nov ;99(5):601-7 PMID: 1358980 |
|---|---|
| 2 | Takao S, Uchikura K, Yonezawa S, Shinchi H, Aikou T. Mucin core protein expression in extrahepatic bile duct carcinoma is associated with metastases to the liver and poor prognosis. Cancer. 1999 Nov 15;86(10):1966-75 PMID: 10570420 |
| 3 | Zenita K, Hirashima K, Shigeta K, Hiraiwa N, Takada A, Hashimoto K, Fujimoto E, Yago K, Kannagi R. Northern hybridization analysis of VH gene expression in murine monoclonal antibodies directed to cancer-associated ganglioside antigens having various sialic acid linkages. J Immunol. 1990 Jun 1;144(11):4442-51 PMID: 2111348 |
| 4 | Fukushi Y, Nudelman E, Levery SB, Hakomori S, Rauvala H. Novel fucolipids accumulating in human adenocarcinoma. III. A hybridoma antibody (FH6) defining a human cancer-associated difucoganglioside (VI3NeuAcV3III3Fuc2nLc6). J Biol Chem. 1984 Aug 25;259(16):10511-7 PMID: 6469974 |
| 5 | Muroi K, Suda T, Nojiri H, Ema H, Amemiya Y, Miura Y, Nakauchi H, Singhal A, Hakomori S. Reactivity profiles of leukemic myeloblasts with monoclonal antibodies directed to sialosyl-Le(x) and other lacto-series type 2 chain antigens:absence of reactivity with normal hematopoietic progenitor cells. Blood. 1992 Feb 1;79(3):713-9 PMID: 1370643 |
| 6 | Dohi T, Abe K, Ohshiba S, Yamaguchi K, Oshima M. Immunohistological and biochemical detection of fucosylated polylactosamine antigen with monoclonal antibody ACFH18 in gastric cancer and normal gastric mucosa. Jpn J Cancer Res. 1990 Jun ;81(6-7):676-81 PMID: 2119368 |
| 7 | Fukushi Y, Kannagi R, Hakomori S, Shepard T, Kulander BG, Singer JW. Location and distribution of difucoganglioside (VI3NeuAcV3III3Fuc2nLc6) in normal and tumor tissues defined by its monoclonal antibody FH6. Cancer Res. 1985 Aug ;45(8):3711-7 PMID: 4016748 |