2D3
| Antibody ID | AN0005 |
|---|
| Epitope ID | EP0008 |
|---|---|
| Epitope name | Sialyl Lewis a |
| Recognition region | Neu5Ac(a2-3)Gal(b1-3)GlcNAc(b1-)-R Neu5Ac(a2-3)Gal(b1-3)[Fuc(a1-4)]GlcNAc(b1-)-R |
| Immunoprecipitation | |
| Immunoblot | [3][1][2][5] |
| ELISA/RIA | [5][4] |
| Flow cytometry | |
| Histochemistry | |
| Comment |
| Epitope ID | EP0041 |
|---|---|
| Epitope name | Sialyl Lewis c |
| Recognition region | Neu5Ac(a2-3)Gal(b1-3)GlcNAc(b1-)-R Neu5Ac(a2-3)Gal(b1-3)[Fuc(a1-4)]GlcNAc(b1-)-R |
| Immunoprecipitation | |
| Immunoblot | [3][1][2][5] |
| ELISA/RIA | [5][4] |
| Flow cytometry | |
| Histochemistry | |
| Comment |
| Species | Mouse |
|---|---|
| Category | Monoclonal |
| Isotype | IgM |
| Immunogen | COLO 201/human colon adenocarcinoma |
| Availability | Tokyo Chemical Industry Co., Ltd. |
| Applications |
| 1 | Takada A, Ohmori K, Yoneda T, Tsuyuoka K, Hasegawa A, Kiso M, Kannagi R. Contribution of carbohydrate antigens sialyl Lewis A and sialyl Lewis X to adhesion of human cancer cells to vascular endothelium. Cancer Res. 1993 Jan 15;53(2):354-61 PMID: 7678075 |
|---|---|
| 2 | Iwai K, Ishikura H, Kaji M, Sugiura H, Ishizu A, Takahashi C, Kato H, Tanabe T, Yoshiki T. Importance of E-selectin (ELAM-1) and sialyl Lewis(a) in the adhesion of pancreatic carcinoma cells to activated endothelium. Int J Cancer. 1993 Jul 30;54(6):972-7 PMID: 7687590 |
| 3 | Takada A, Ohmori K, Takahashi N, Tsuyuoka K, Yago A, Zenita K, Hasegawa A, Kannagi R. Adhesion of human cancer cells to vascular endothelium mediated by a carbohydrate antigen, sialyl Lewis A. Biochem Biophys Res Commun. 1991 Sep 16;179(2):713-9 PMID: 1716885 |
| 4 | Zenita K, Hirashima K, Shigeta K, Hiraiwa N, Takada A, Hashimoto K, Fujimoto E, Yago K, Kannagi R. Northern hybridization analysis of VH gene expression in murine monoclonal antibodies directed to cancer-associated ganglioside antigens having various sialic acid linkages. J Immunol. 1990 Jun 1;144(11):4442-51 PMID: 2111348 |
| 5 | Kannagi R, Takada A, Shigeta K, Itai S, Hashimoto K. [2D3 series, MSW 113 and other monoclonal antibodies directed to type 1 chain carbohydrate antigens] Nippon Rinsho. 1990 Feb ;48 Suppl:992-8 PMID: 2355641 |