R24
| Antibody ID | AN0417 |
|---|
| Epitope ID | EP0062 |
|---|---|
| Epitope name | N-Acetyl GD3 |
| Recognition region | Neu5Ac(a2-8)Neu5Ac(a2-3)Gal-R Neu5Ac(a2-8)Neu5Gc(a2-3)Gal-R |
| Immunoprecipitation | |
| Immunoblot | [1][2] |
| ELISA/RIA | [2][3][4] |
| Flow cytometry | |
| Histochemistry | |
| Comment | Reacted to sulfatide.[4] Not reacted to GD1b and GT1b.[5] Gal in the recognition region should not have branched structure.[5] |
| Epitope ID | EP0306 |
|---|---|
| Epitope name | Neu5Ac(a2-8)Neu5Gc(a2-3)Gal |
| Recognition region | Neu5Ac(a2-8)Neu5Ac(a2-3)Gal-R Neu5Ac(a2-8)Neu5Gc(a2-3)Gal-R |
| Immunoprecipitation | |
| Immunoblot | [2] |
| ELISA/RIA | [2] |
| Flow cytometry | |
| Histochemistry | |
| Comment | Reacted to sulfatide.[4] Not reacted to GD1b and GT1b.[5] Gal in the recognition region should not have branched structure.[5] |
| Epitope ID | EP0308 |
|---|---|
| Epitope name | Neu5Ac(a2-8)Neu5Ac(a2-3)Gal |
| Recognition region | Neu5Ac(a2-8)Neu5Ac(a2-3)Gal-R Neu5Ac(a2-8)Neu5Gc(a2-3)Gal-R |
| Immunoprecipitation | |
| Immunoblot | [2] |
| ELISA/RIA | [2][3][5][4] |
| Flow cytometry | |
| Histochemistry | |
| Comment | Reacted to sulfatide.[4] Not reacted to GD1b and GT1b.[5] Gal in the recognition region should not have branched structure.[5] |
| Species | Mouse |
|---|---|
| Category | Monoclonal |
| Isotype | IgG3 |
| Immunogen | SK-MEL-28/human melanoma[6] |
| Availability | abcam Affinity BioReagents GENETEX,Inc. GenWay Biotech Inc. Thermo Scientific |
| Applications |
| 1 | Pukel CS, Lloyd KO, Travassos LR, Dippold WG, Oettgen HF, Old LJ. GD3, a prominent ganglioside of human melanoma. Detection and characterisation by mouse monoclonal antibody. J Exp Med. 1982 Apr 1;155(4):1133-47 PMID: 7061953 |
|---|---|
| 2 | Tai T, Kawashima I, Furukawa K, Lloyd KO. Monoclonal antibody R24 distinguishes between different N-acetyl- and N-glycolylneuraminic acid derivatives of ganglioside GD3. Arch Biochem Biophys. 1988 Jan ;260(1):51-5 PMID: 2449128 |
| 3 | Hildebrandt H. Antigen binding of antiganglioside antibodies in vitro is strongly influenced by the ganglioside composition of the sample. FEBS Lett. 1996 Jun 10;388(1):29-33 PMID: 8654583 |
| 4 | Townson K, Greenshields KN, Veitch J, Nicholl D, Eckhardt M, Galanina O, Bovin N, Samain E, Antoine T, Bundle D, Zhang P, Ling CC, Willison HJ. Sulfatide binding properties of murine and human antiganglioside antibodies. Glycobiology. 2007 Nov ;17(11):1156-66 PMID: 17855742 |
| 5 | Willison HJ, Townson K, Veitch J, Boffey J, Isaacs N, Andersen SM, Zhang P, Ling CC, Bundle DR. Synthetic disialylgalactose immunoadsorbents deplete anti-GQ1b antibodies from autoimmune neuropathy sera. Brain. 2004 Mar ;127(Pt 3):680-91 PMID: 14960498 |
| 6 | Dippold WG, Lloyd KO, Li LT, Ikeda H, Oettgen HF, Old LJ. Cell surface antigens of human malignant melanoma: definition of six antigenic systems with mouse monoclonal antibodies. Proc Natl Acad Sci U S A. 1980 Oct ;77(10):6114-8 PMID: 6934537 |