Antibody IDAN0269
Epitope ID EP0500
Epitope nameHomogalacturonan
Recognition regionMeGalA(a1-4)GalA(a1-4)GalA(a1-4)GalA(a1-4)GalA(a1-4)MeGalA -R
Flow cytometry
CommentThe JIM5 optimally binds to at least four contiguous unesterified residues between or adjacent to a metyl-ester group.[3]

JIM5 binds to unesterified GalA residues with adjacent or flanking metyl-esterified residues.[3]
Plant Probes
ApplicationsImmunodot assay[1][7][5][6]
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1 Willats WG, Limberg G, Buchholt HC, van Alebeek GJ, Benen J, Christensen TM, Visser J, Voragen A, Mikkelsen JD, Knox JP.
Analysis of pectic epitopes recognised by hybridoma and phage display monoclonal antibodies using defined oligosaccharides, polysaccharides, and enzymatic degradation.
Carbohydr Res. 2000 Jul 24;327(3):309-20
PMID: 10945679
2 Verhertbruggen Y, Marcus SE, Haeger A, Ordaz-Ortiz JJ, Knox JP.
An extended set of monoclonal antibodies to pectic homogalacturonan.
Carbohydr Res. 2009 Sep 28;344(14):1858-62
PMID: 19144326
3 Clausen MH, Willats WG, Knox JP.
Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7.
Carbohydr Res. 2003 Aug 12;338(17):1797-800
PMID: 12892947
4 Marcus SE, Blake AW, Benians TA, Lee KJ, Poyser C, Donaldson L, Leroux O, Rogowski A, Petersen HL, Boraston A, Gilbert HJ, Willats WG, Knox JP.
Restricted access of proteins to mannan polysaccharides in intact plant cell walls.
Plant J. 2010 Oct ;64(2):191-203
PMID: 20659281
5 Willats WG, Steele-King CG, Marcus SE, Knox JP.
Side chains of pectic polysaccharides are regulated in relation to cell proliferation and cell differentiation
Plant J. 1999 Dec ;20(6):619-28
PMID: 10652134
6 Orfila C, Knox JP.
Spatial regulation of pectic polysaccharides in relation to pit fields in cell walls of tomato fruit pericarp.
Plant Physiol. 2000 Mar ;122(3):775-81
PMID: 10712541
7 Willats WG, Orfila C, Limberg G, Buchholt HC, van Alebeek GJ, Voragen AG, Marcus SE, Christensen TM, Mikkelsen JD, Murray BS, Knox JP.
Modulation of the degree and pattern of methyl-esterification of pectic homogalacturonan in plant cell walls. Implications for pectin methyl esterase action, matrix properties, and cell adhesion.
J Biol Chem. 2001 Jun 1;276(22):19404-13
PMID: 11278866
8 Lee KJ, Sakata Y, Mau SL, Pettolino F, Bacic A, Quatrano RS, Knight CD, Knox JP.
Arabinogalactan proteins are required for apical cell extension in the moss Physcomitrella patens.
Plant Cell. 2005 Nov ;17(11):3051-65
PMID: 16199618
9 Marcus SE, Verhertbruggen Y, Hervé C, Ordaz-Ortiz JJ, Farkas V, Pedersen HL, Willats WG, Knox JP.
Pectic homogalacturonan masks abundant sets of xyloglucan epitopes in plant cell walls.
BMC Plant Biol. 2008 May 22;8:60
PMID: 18498625
10 Moller I, Marcus SE, Haeger A, Verhertbruggen Y, Verhoef R, Schols H, Ulvskov P, Mikkelsen JD, Knox JP, Willats W.
High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray binding profiles.
Glycoconj J. 2008 Jan ;25(1):37-48
PMID: 17629746