JIM7
| Antibody ID | AN0270 |
|---|
| Epitope ID | EP0500 |
|---|---|
| Epitope name | Homogalacturonan |
| Recognition region | GalA(a1-4)MeGalA(a1-4)MeGalA(a1-4)MeGalA(a1-4)MeGalA(a1-4)GalA(a1-4)-R GalA(a1-4)GalA(a1-4)MeGalA(a1-4)MeGalA(a1-4)MeGalA(a1-4)GalA(a1-4)-R MeGalA(a1-4)GalA(a1-4)MeGalA(a1-4)GalA(a1-4)MeGalA(a1-4)GalA(a1-4)-R |
| Immunoprecipitation | |
| Immunoblot | |
| ELISA/RIA | [1][4][3][5] |
| Flow cytometry | |
| Histochemistry | [2][3] |
| Comment | JIM7 binds to metyl-esterified residues with adjacent or flanking unesterified GalA residues.[4] |
| Species | Rat |
|---|---|
| Category | Monoclonal |
| Isotype | IgA |
| Immunogen | |
| Availability | CarboSouse |
| Applications | Immunodot assay[1][2] |
| 1 | Willats WG, Limberg G, Buchholt HC, van Alebeek GJ, Benen J, Christensen TM, Visser J, Voragen A, Mikkelsen JD, Knox JP. Analysis of pectic epitopes recognised by hybridoma and phage display monoclonal antibodies using defined oligosaccharides, polysaccharides, and enzymatic degradation. Carbohydr Res. 2000 Jul 24;327(3):309-20 PMID: 10945679 |
|---|---|
| 2 | McCartney L, Ormerod AP, Gidley MJ, Knox JP. Temporal and spatial regulation of pectic (1-->4)-beta-D-galactan in cell walls of developing pea cotyledons: implications for mechanical properties. Plant J. 2000 Apr ;22(2):105-13 PMID: 10792826 |
| 3 | Verhertbruggen Y, Marcus SE, Haeger A, Ordaz-Ortiz JJ, Knox JP. An extended set of monoclonal antibodies to pectic homogalacturonan. Carbohydr Res. 2009 Sep 28;344(14):1858-62 PMID: 19144326 |
| 4 | Clausen MH, Willats WG, Knox JP. Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr Res. 2003 Aug 12;338(17):1797-800 PMID: 12892947 |
| 5 | Marcus SE, Blake AW, Benians TA, Lee KJ, Poyser C, Donaldson L, Leroux O, Rogowski A, Petersen HL, Boraston A, Gilbert HJ, Willats WG, Knox JP. Restricted access of proteins to mannan polysaccharides in intact plant cell walls. Plant J. 2010 Oct ;64(2):191-203 PMID: 20659281 |