LM7
| Antibody ID | AN0355 |
|---|
| Epitope ID | EP0500 |
|---|---|
| Epitope name | Homogalacturonan |
| Recognition region | partially Me-Homogalacturonan/ non-blockwise |
| Immunoprecipitation | |
| Immunoblot | |
| ELISA/RIA | [2][1] |
| Flow cytometry | |
| Histochemistry | |
| Comment | LM7 binds to unesterified GalA residues with adjacent or flanking metyl-esterified residues.[1] |
| Species | Rat |
|---|---|
| Category | Monoclonal |
| Isotype | IgM |
| Immunogen | |
| Availability | Plant Probes |
| Applications | Immunodot assay[2] |
| 1 | Clausen MH, Willats WG, Knox JP. Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr Res. 2003 Aug 12;338(17):1797-800 PMID: 12892947 |
|---|---|
| 2 | Willats WG, Orfila C, Limberg G, Buchholt HC, van Alebeek GJ, Voragen AG, Marcus SE, Christensen TM, Mikkelsen JD, Murray BS, Knox JP. Modulation of the degree and pattern of methyl-esterification of pectic homogalacturonan in plant cell walls. Implications for pectin methyl esterase action, matrix properties, and cell adhesion. J Biol Chem. 2001 Jun 1;276(22):19404-13 PMID: 11278866 |