GlycoEpitope: show

Sialyl Lewis x

Epitope information of EP0012

Epitope ID	EP0012
Epitope name	Sialyl Lewis x
Structure
Sequence	Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-)-R
Aliases	Sialyl Le^x,CD15s,sCD15
History	Sialyl Le^x was charaterized as a tumor marker. [30][31][32][33] The term Lewis refers to the family name of individuals suffering from a red blood cell incompatibility problem that helped lead to the discovery of this blood group.
DB	GlyTouCan:G00054MO JCGGDB
Molecular weight	982.9
Composition	(Fuc)1(Gal)2(GlcNAc)1(Neu5Ac)1
Species	Homo sapiens Mus musculus
Tissue and Cellular distribution	cancer/colon [16] helper memory T cell hematopoietic stem cell leukocyte(human)[34][35] monocyte neutrophil NK cell normal epithelia/breast[52] normal epithelia/colon[52] normal epithelia/oral cavity[52]
Subcellular distribution
Developmental change
Cell line	BxPC-3/human pancreatic carcinoma COLO 205/human colon adenocarcinoma HL-60/human promyelocytic leukemia HT-29/human colon adenocarcinoma HT-29LMM/metastatic parental
Receptor	E-selectin L-selectin(The hierarchy of binding strengths were 6-sulfo sialyl > sialyl = 6',6-bis-sulfo sialyl >> 6'-sulfo sialyl Le^x)[41] P-selectin(requires PSGL-1 molecules carrying sialyl Le^x and presence of sulfated tyrosine residue(s) in its N-terminal region)
Function	Sialyl Le^x was shown to be a specific ligand on human leukocytes for vascular E- and P-selectins,and was shown to mediate leukocyte recruitment .[30][31][32] The determinant is also frequently expressed on human cancer cells,and is involved in the adhesion of cancer cells to vascular endothelium and thus contribute to hematogenous metastasis of cancer.[17]
Diseases	colon cancer[16] colorectal cancer[53] The prognosis of patients in whom type 2 carbohydrate antigens were increased in the cancerous portion, especially Le^x and sialyl Le^x-i, was poorer than in patients in whom they were not increased.[22][23]
Application	The monoclonal antibodies,CSLEX-1,NCC-ST-439 and FH-6 are representative diagnostic agents for adenocarcinoma.
Comment

Epitope ID

EP0012

Epitope name

Sialyl Lewis x

Structure

Sequence

Neu5Ac(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-)-R

Aliases

Sialyl Le^x,CD15s,sCD15

History

Sialyl Le^x was charaterized as a tumor marker. [30][31][32][33]
The term Lewis refers to the family name of individuals suffering from a red blood cell incompatibility problem that helped lead to the discovery of this blood group.

GlyTouCan:G00054MO JCGGDB

Molecular weight

982.9

Composition

(Fuc)1(Gal)2(GlcNAc)1(Neu5Ac)1

Species

Homo sapiens
Mus musculus

Tissue and Cellular distribution

cancer/colon [16]
helper memory T cell
hematopoietic stem cell
leukocyte(human)[34][35]
monocyte
neutrophil
NK cell
normal epithelia/breast[52]
normal epithelia/colon[52]
normal epithelia/oral cavity[52]

Subcellular distribution

Developmental change

Cell line

BxPC-3/human pancreatic carcinoma
COLO 205/human colon adenocarcinoma
HL-60/human promyelocytic leukemia
HT-29/human colon adenocarcinoma
HT-29LMM/metastatic parental

Receptor

E-selectin
L-selectin(The hierarchy of binding strengths were 6-sulfo sialyl > sialyl = 6',6-bis-sulfo sialyl >> 6'-sulfo sialyl Le^x)[41]
P-selectin(requires PSGL-1 molecules carrying sialyl Le^x and presence of sulfated tyrosine residue(s) in its N-terminal region)

Function

Sialyl Le^x was shown to be a specific ligand on human leukocytes for vascular E- and P-selectins,and was shown to mediate leukocyte recruitment .[30][31][32]
The determinant is also frequently expressed on human cancer cells,and is involved in the adhesion of cancer cells to vascular endothelium and thus contribute to hematogenous metastasis of cancer.[17]

Diseases

colon cancer[16]
colorectal cancer[53]

The prognosis of patients in whom type 2 carbohydrate antigens were increased in the cancerous portion, especially Le^x and sialyl Le^x-i, was poorer than in patients in whom they were not increased.[22][23]

Application

The monoclonal antibodies,CSLEX-1,NCC-ST-439 and FH-6 are representative diagnostic agents for adenocarcinoma.

Comment