MLS128
| Antibody ID | AN0080 |
|---|
| Epitope ID | EP0021 |
|---|---|
| Epitope name | Tn Antigen |
| Recognition region | GalNAc(a1-)Ser/Thr |
| Immunoprecipitation | [2] |
| Immunoblot | [2][1][4][7] |
| ELISA/RIA | [5] |
| Flow cytometry | [7] |
| Histochemistry | [3][6] |
| Comment | MLS128 binds to a carbohydrate epitope consisting of three consecutive Tn-antigens (GalNAcalpha-Ser/Thr). [8][7] |
| Species | Mouse |
|---|---|
| Category | Monoclonal |
| Isotype | IgG3 |
| Immunogen | |
| Availability | Wako Pure Chemical Industries, Ltd. |
| Applications | A radiolabeled -Protein A binding assay to an IgG antibody bound on an antigen-coated plate[8] |
| 1 | Nakada H, Inoue M, Tanaka N, Yamashina I. Coexpression of cancer-associated carbohydrate antigens, Tn and sialyl Tn. Glycoconj J. 1994 Jun ;11(3):262-5 PMID: 7841802 |
|---|---|
| 2 | Inoue M, Nakada H, Tanaka N, Yamashina I. Tn antigen is expressed on leukosialin from T-lymphoid cells. Cancer Res. 1994 Jan 1;54(1):85-8 PMID: 8261467 |
| 3 | Ohshio G, Imamura T, Imamura M, Yamabe H, Sakahara H, Nakada H, Yamashina I. Distribution of Tn antigen recognized by an anti-Tn monoclonal antibody (MLS128) in normal and malignant tissues of the digestive tract. J Cancer Res Clin Oncol. 1995 ;121(4):247-52 PMID: 7751324 |
| 4 | Toda M, Hisano R, Yurugi H, Akita K, Maruyama K, Inoue M, Adachi T, Tsubata T, Nakada H. Ligation of tumour-produced mucins to CD22 dramatically impairs splenic marginal zone B-cells. Biochem J. 2009 Feb 1;417(3):673-83 PMID: 18925876 |
| 5 | Freire T, Bay S, von Mensdorff-Pouilly S, Osinaga E. Molecular basis of incomplete O-glycan synthesis in MCF-7 breast cancer cells: putative role of MUC6 in Tn antigen expression. Cancer Res. 2005 Sep 1;65(17):7880-7 PMID: 16140958 |
| 6 | Toda M, Akita K, Inoue M, Taketani S, Nakada H. Down-modulation of B cell signal transduction by ligation of mucins to CD22. Biochem Biophys Res Commun. 2008 Jul 18;372(1):45-50 PMID: 18474217 |
| 7 | Morita N, Yajima Y, Asanuma H, Nakada H, Fujita-Yamaguchi Y. Inhibition of cancer cell growth by anti-Tn monoclonal antibody MLS128. Biosci Trends. 2009 Feb ;3(1):32-7 PMID: 20103942 |
| 8 | Nakada H, Inoue M, Numata Y, Tanaka N, Funakoshi I, Fukui S, Mellors A, Yamashina I. Epitopic structure of Tn glycophorin A for an anti-Tn antibody (MLS 128). Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2495-9 PMID: 7681597 |