PAM1
| Antibody ID | AN0271 |
|---|
| Epitope ID | EP0500 |
|---|---|
| Epitope name | Homogalacturonan |
| Recognition region | blockwise de-esterifield Homogalacturonan |
| Immunoprecipitation | |
| Immunoblot | |
| ELISA/RIA | [1] |
| Flow cytometry | |
| Histochemistry | [1][3] |
| Comment | Optimal binding required in the region of 30 de-estrified GalA residues.[1] |
| Species | - |
|---|---|
| Category | Single chain Fv |
| Isotype | |
| Immunogen | |
| Availability | Plant Probes |
| Applications | Immunodot assay [1][2][3] Microarray[4] |
| 1 | Willats WG, Gilmartin PM, Mikkelsen JD, Knox JP. Cell wall antibodies without immunization: generation and use of de-esterified homogalacturonan block-specific antibodies from a naive phage display library. Plant J. 1999 Apr ;18(1):57-65 PMID: 10341443 |
|---|---|
| 2 | Willats WG, Limberg G, Buchholt HC, van Alebeek GJ, Benen J, Christensen TM, Visser J, Voragen A, Mikkelsen JD, Knox JP. Analysis of pectic epitopes recognised by hybridoma and phage display monoclonal antibodies using defined oligosaccharides, polysaccharides, and enzymatic degradation. Carbohydr Res. 2000 Jul 24;327(3):309-20 PMID: 10945679 |
| 3 | Willats WG, Orfila C, Limberg G, Buchholt HC, van Alebeek GJ, Voragen AG, Marcus SE, Christensen TM, Mikkelsen JD, Murray BS, Knox JP. Modulation of the degree and pattern of methyl-esterification of pectic homogalacturonan in plant cell walls. Implications for pectin methyl esterase action, matrix properties, and cell adhesion. J Biol Chem. 2001 Jun 1;276(22):19404-13 PMID: 11278866 |
| 4 | Moller I, Marcus SE, Haeger A, Verhertbruggen Y, Verhoef R, Schols H, Ulvskov P, Mikkelsen JD, Knox JP, Willats W. High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray binding profiles. Glycoconj J. 2008 Jan ;25(1):37-48 PMID: 17629746 |