LM6
| Antibody ID | AN0289 |
|---|
| Epitope ID | EP0508 |
|---|---|
| Epitope name | Arabinan |
| Recognition region | -[Ara(a1-5)]n- n=5 |
| Immunoprecipitation | |
| Immunoblot | [3][4][8][5] |
| ELISA/RIA | [1][7] |
| Flow cytometry | |
| Histochemistry | [7][3][4][2][8][5][6] |
| Comment | The series of (1-5)-alpha-L-arabinobiose to (1-5)-alpha-L-arabinopentaose displayed increaseing capacity to inhibit LM6 binding in the competitive inhibition assays with the to arabinohexaose being the most effectinv inhibitor indicating that the size of the epitope is 5-6 (1-5)-alpha-L-linked arabinose residues.[1] |
| Species | Rat |
|---|---|
| Category | Monoclonal |
| Isotype | IgG |
| Immunogen | |
| Availability | Plant Probes |
| Applications | Immunodot assay[1][3][4][2][8] |
| 1 | Willats WG, Marcus SE, Knox JP. Generation of monoclonal antibody specific to (1-->5)-alpha-L-arabinan. Carbohydr Res. 1998 Mar ;308(1-2):149-52 PMID: 9675359 |
|---|---|
| 2 | McCartney L, Ormerod AP, Gidley MJ, Knox JP. Temporal and spatial regulation of pectic (1-->4)-beta-D-galactan in cell walls of developing pea cotyledons: implications for mechanical properties. Plant J. 2000 Apr ;22(2):105-13 PMID: 10792826 |
| 3 | Willats WG, Steele-King CG, Marcus SE, Knox JP. Side chains of pectic polysaccharides are regulated in relation to cell proliferation and cell differentiation Plant J. 1999 Dec ;20(6):619-28 PMID: 10652134 |
| 4 | Orfila C, Knox JP. Spatial regulation of pectic polysaccharides in relation to pit fields in cell walls of tomato fruit pericarp. Plant Physiol. 2000 Mar ;122(3):775-81 PMID: 10712541 |
| 5 | Lee KJ, Sakata Y, Mau SL, Pettolino F, Bacic A, Quatrano RS, Knight CD, Knox JP. Arabinogalactan proteins are required for apical cell extension in the moss Physcomitrella patens. Plant Cell. 2005 Nov ;17(11):3051-65 PMID: 16199618 |
| 6 | Marcus SE, Verhertbruggen Y, Hervé C, Ordaz-Ortiz JJ, Farkas V, Pedersen HL, Willats WG, Knox JP. Pectic homogalacturonan masks abundant sets of xyloglucan epitopes in plant cell walls. BMC Plant Biol. 2008 May 22;8:60 PMID: 18498625 |
| 7 | Verhertbruggen Y, Marcus SE, Haeger A, Verhoef R, Schols HA, McCleary BV, McKee L, Gilbert HJ, Knox JP. Developmental complexity of arabinan polysaccharides and their processing in plant cell walls. Plant J. 2009 Aug ;59(3):413-25 PMID: 19392693 |
| 8 | Orfila C, Seymour GB, Willats WG, Huxham IM, Jarvis MC, Dover CJ, Thompson AJ, Knox JP. Altered middle lamella homogalacturonan and disrupted deposition of (1-->5)-alpha-L-arabinan in the pericarp of Cnr, a ripening mutant of tomato. Plant Physiol. 2001 May ;126(1):210-21 PMID: 11351084 |